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Valiant Co Ltd anti β galactosidase
Anti β Galactosidase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2+galactosidase/%CE%B2-Galactosidase/pm42000019-133-11-13
Average 96 stars, based on 292 article reviews
anti β galactosidase - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Staining:

Article Title: Stem cell growth directs region-specific cell fate decisions during intestinal nutrient adaptation
Article Snippet: .. Tissues were washed with 0.1% Triton-X 100 in PBS and blocked in 1% bovine serum albumin for 1 h. Subsequently, tissues were stained with anti-β-Galactosidase (1:400) (MP Biomedicals cat no: 0855976-CF), anti-Prospero (1:1000) (MR1A, DSHB), anti-p4EBP (CST 2855) antibodies. .. The samples were mounted in Vectashield mounting media with DAPI (Vector Laboratories) and imaged using the Aurox clarity confocal system (Aurox).

Article Title: Stem cell mTOR signaling directs region-specific cell fate decisions during intestinal nutrient adaptation
Article Snippet: .. Subsequently, tissues were stained with anti–β-galactosidase (1:400) (MP Biomedicals, catalog no. 0855976-CF), anti-Prospero (1:1000) (Developmental Studies Hybridoma Bank, MR1A), anti-p4EBP (Cell Signaling Technology, 2855) antibodies. .. The samples were mounted in Vectashield mounting medium with DAPI (Vector Laboratories).

Article Title: Nuclear Factor Y controls epithelial growth by regulating mTOR in the Drosophila midgut
Article Snippet: .. The fixed tissue was then washed with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 h. Subsequently, intestines were stained with anti-NF-YA (1:400) (gift from Hideki Yoshida, Kyoto Institute of Technology), anti-β-galactosidase (1:400) (MP Biomedicals 0855976-CF), anti-Prospero (1:1000) (Developmental Studies Hybridoma Bank MR1A), anti-Dlg (1:50) (Developmental Studies Hybridoma Bank 4F3), anti-Armadillo (1:20) (Developmental Studies Hybridoma Bank N27A1), anti-p4EBP (1:400) (Cell signaling 2855), anti-Horseradish Peroxidase HRP 1:200 (Jackson ImmunoResearch Laboratories 2338967), anti-TOM20 1:400 (BD Biosciences, 612278) and Phalloidin 1:300 (Invitrogen A12380). .. Samples were mounted in Vectashield with DAPI (Vector Laboratories).

Article Title: Metabolic gene regulation by Drosophila GATA transcription factor Grain
Article Snippet: .. Midguts were washed with 0.1% Triton-X 100 in PBS and blocked in 1% bovine serum albumin for 1 h. Midguts were then stained with anti-β-Galactosidase (1:1000) antibody (MP Biomedicals) overnight in +4°C, then washed 4 times and stained with anti-rabbit alexa 568 (1:1000) (Life Technologies) for 2 hours. .. Midguts were again washed 4 times, and were then mounted in Vectashield Mounting Media with DAPI (Vector Laboratories).

other:

Article Title: High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothened
Article Snippet: Antibody , anti-β-Galactosidase (rabbit polyclonal) , MP Biomedicals , 085597-CF , IF(1:100).

Article Title: Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgut
Article Snippet: Antibody , anti-β-Galactosidase (rabbit polyclonal) , MP Biomedicals , Cat#: 08559761 RRID: AB_3675281 , IF (1:1000).

Tissue:

Article Title: Nuclear Factor Y controls epithelial growth by regulating mTOR in the Drosophila midgut
Article Snippet: .. The fixed tissue was then washed with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 h. Subsequently, intestines were stained with anti-NF-YA (1:400) (gift from Hideki Yoshida, Kyoto Institute of Technology), anti-β-galactosidase (1:400) (MP Biomedicals 0855976-CF), anti-Prospero (1:1000) (Developmental Studies Hybridoma Bank MR1A), anti-Dlg (1:50) (Developmental Studies Hybridoma Bank 4F3), anti-Armadillo (1:20) (Developmental Studies Hybridoma Bank N27A1), anti-p4EBP (1:400) (Cell signaling 2855), anti-Horseradish Peroxidase HRP 1:200 (Jackson ImmunoResearch Laboratories 2338967), anti-TOM20 1:400 (BD Biosciences, 612278) and Phalloidin 1:300 (Invitrogen A12380). .. Samples were mounted in Vectashield with DAPI (Vector Laboratories).

Cell-Signaling:

Article Title: Nuclear Factor Y controls epithelial growth by regulating mTOR in the Drosophila midgut
Article Snippet: .. The fixed tissue was then washed with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 h. Subsequently, intestines were stained with anti-NF-YA (1:400) (gift from Hideki Yoshida, Kyoto Institute of Technology), anti-β-galactosidase (1:400) (MP Biomedicals 0855976-CF), anti-Prospero (1:1000) (Developmental Studies Hybridoma Bank MR1A), anti-Dlg (1:50) (Developmental Studies Hybridoma Bank 4F3), anti-Armadillo (1:20) (Developmental Studies Hybridoma Bank N27A1), anti-p4EBP (1:400) (Cell signaling 2855), anti-Horseradish Peroxidase HRP 1:200 (Jackson ImmunoResearch Laboratories 2338967), anti-TOM20 1:400 (BD Biosciences, 612278) and Phalloidin 1:300 (Invitrogen A12380). .. Samples were mounted in Vectashield with DAPI (Vector Laboratories).



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Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated <t>β-galactosidase</t> <t>(SA-β-gal)</t> activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05.
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Image Search Results


Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated β-galactosidase (SA-β-gal) activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated β-galactosidase (SA-β-gal) activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Inhibition, Expressing, Immunohistochemical staining, Staining, Western Blot, Immunohistochemistry, Activity Assay

Inhibition of Numb promoted mitotic arrest and cellular senescence after radiation in colonic cells. (A) Flow cytometry was utilized to assess the apoptosis of HCT116 cells following 24 h and 72 h of radiation. n = 3 repeats in each group. (B) Flow cytometry was utilized to analyze the cell cycle distribution of colonic HCT116 cells at 24 h and 72 h postradiation. ** P < 0.01 IR versus IR+shNumb group at 24 h, * P < 0.05 IR versus IR+shNumb group at 72 h. n = 3 repeats in each group. (C) Immunofluorescence staining was conducted to evaluate the expression of the DNA damage marker γH2AX after 72 h of radiation. Scale bar = 50 μm. (D) Cell senescence-associated β-gal staining was performed to analyze cell senescence after 72 h of radiation. Scale bar = 50 μm.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: Inhibition of Numb promoted mitotic arrest and cellular senescence after radiation in colonic cells. (A) Flow cytometry was utilized to assess the apoptosis of HCT116 cells following 24 h and 72 h of radiation. n = 3 repeats in each group. (B) Flow cytometry was utilized to analyze the cell cycle distribution of colonic HCT116 cells at 24 h and 72 h postradiation. ** P < 0.01 IR versus IR+shNumb group at 24 h, * P < 0.05 IR versus IR+shNumb group at 72 h. n = 3 repeats in each group. (C) Immunofluorescence staining was conducted to evaluate the expression of the DNA damage marker γH2AX after 72 h of radiation. Scale bar = 50 μm. (D) Cell senescence-associated β-gal staining was performed to analyze cell senescence after 72 h of radiation. Scale bar = 50 μm.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Inhibition, Flow Cytometry, Immunofluorescence, Staining, Expressing, Marker

PLK1 overexpression compensated for the Numb knockdown-induced cellular senescence phenotype in colonic cells after radiation. (A) Senescence-associated β-gal staining was performed on different groups of HCT116 cells. The results of the quantitative analysis of the percentage of cells with positive β-gal staining are shown in the right panel. Scale bar = 50 μm. (B) Colony formation assay illustrating colonies in different groups of HCT116 cells after radiation. Quantitative analysis of the total optical density of the colony formation assay is shown in the right panel. (C-E) RT‒PCR was used to detect the expression of the indicated cytokines (IL-6, IL-8, and IL-1β) in different groups of cells. The expression levels were normalized to those of β-actin. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05. n = 3 repeats in each group.

Journal: Scientific Reports

Article Title: Numb mitigates intestinal epithelial cell senescence induced by radiation through a PLK1-dependent pathway

doi: 10.1038/s41598-026-44793-x

Figure Lengend Snippet: PLK1 overexpression compensated for the Numb knockdown-induced cellular senescence phenotype in colonic cells after radiation. (A) Senescence-associated β-gal staining was performed on different groups of HCT116 cells. The results of the quantitative analysis of the percentage of cells with positive β-gal staining are shown in the right panel. Scale bar = 50 μm. (B) Colony formation assay illustrating colonies in different groups of HCT116 cells after radiation. Quantitative analysis of the total optical density of the colony formation assay is shown in the right panel. (C-E) RT‒PCR was used to detect the expression of the indicated cytokines (IL-6, IL-8, and IL-1β) in different groups of cells. The expression levels were normalized to those of β-actin. The values are presented as the means ± SEMs. ** P < 0.01, * P < 0.05. n = 3 repeats in each group.

Article Snippet: After blocking with TBST containing 5% nonfat dry milk, specific primary antibodies against Numb (Abcam, Ab220362 ), p53 (Abcam, Ab131442 ), p21 (Santa Cruz, sc-377515), p16 (Abcam, ab211542), γH2AX (Santa Cruz, sc-517348), Cyclin B (Santa Cruz, sc-166210), Plk1 (Abcam, ab12157) and β-galactosidase (CST, #27198) were incubated with the membranes at 4 °C overnight, followed by incubation with the respective secondary antibodies.

Techniques: Over Expression, Knockdown, Staining, Colony Assay, Expressing